Coding

Part:BBa_K3425044:Design

Designed by: Nuria Garriga Alonso   Group: iGEM20_UofUppsala   (2020-10-26)


ehaA β-domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 555
    Illegal AgeI site found at 144
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for E. coli.

This part is specifically designed for the agglutination construct of NANOFLEX with custom Type IIS fusion sites compatible with the iGEM Type IIS standard. Visit the Main page for more details.

Source

Taken from Salema, V., Marín, E., Martínez-Arteaga, R., Ruano-Gallego, D., Fraile, S., Margolles, Y., Teira, X., Gutierrez, C., Bodelón, C. & Fernández, L. Á. (2013). Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies. PloS one, 8(9), e75126.

References